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dc.contributor.creatorStack, Julianne
dc.contributor.creatorLe Gouic, Aurélien V.
dc.contributor.creatorTobin, Paul R.
dc.contributor.creatorGuihéneuf, Freddy
dc.contributor.creatorStengel, Dagmar B.
dc.contributor.creatorFitzGerald, Richard J.
dc.date.accessioned2019-02-26T14:53:27Z
dc.date.available2019-02-26T14:53:27Z
dc.date.issued2018
dc.identifier.citationStack, J., Le Gouic, A.V., Tobin, P.R., Guihéneuf, F., Stengel, D.B., and FitzGerald, R.J. (2018). Protein extraction and bioactive hydrolysate generation from two microalgae, Porphyridium purpureum and Phaeodactylum tricornutum. J. Food Bioact. 1: 153–165.en_US
dc.identifier.urihttp://hdl.handle.net/10395/2683
dc.descriptionProtein extraction and bioactive hydrolysate generation from two microalgae, Porphyridium purpureum and Phaeodactylum tricornutum.en_US
dc.description.abstractMicroalgae are a relatively underutilised source of valuable nutritional compounds and biochemicals. Their high protein contents make them an interesting target for the generation of bioactive peptides for the functional food industry. Here, the nitrogenous components of the red microalga Porphyridium purpureum and the diatom-Phaeodactylum tricornutum were quantified, and their SDS-PAGE profiles analysed. Proteinswere isolated from these species by solubilisation at alkaline pH followed by precipitation at acidic pH. The protein extracts were hydrolysed with the food-grade proteolytic preparations Alcalase 2.4 L and Flavourzyme 500 L. The P. purpureum and P. tricornutum hydrolysates were examined for their in vitroanti-diabetic and antioxidant properties. The microalgal-derived protein hydrolysates had dipeptidyl peptidase (DPP) IV inhibitory activity, with IC50 values of 2.28 ± 0.36 and 2.68 ± 0.19 mg/mL for P. purpureum and P. tricornutum, respectively. The oxygen radical absorbance capacity (ORAC) and ferric reducing power (FRAP) values were, respectively, 13.98 ± 0.97 and 478.94 ± 34.43 for P. purpureum, and 15.04 ± 0.54 and 155.74 ± 38.30 for P. tricornutum expressed in μmol Trolox Equivalent (TE) per gram of dry matter.The results suggest that protein hydrolysates from these microalgae have potential use as functional food ingredients.en_US
dc.language.isoengen_US
dc.publisherInternational Society for Nutraceuticals and Functional Foodsen_US
dc.relation.ispartofseries1;
dc.rights.urihttp://www.isnff-jfb.com/index.php/JFB/article/view/15/36en_US
dc.subjectPorphyridium purpureumen_US
dc.subjectPhaeodactylum tricornutumen_US
dc.subjectBioactive peptidesen_US
dc.subjectProtein hydrolysatesen_US
dc.subjectAntioxidanten_US
dc.subjectDPP-IVen_US
dc.titleProtein extraction and bioactive hydrolysate generation from two microalgae, Porphyridium purpureum and Phaeodactylum tricornutumen_US
dc.typeArticleen_US
dc.type.supercollectionall_mic_researchen_US
dc.type.supercollectionmic_published_revieweden_US
dc.description.versionYesen_US
dc.identifier.doi10.31665/JFB.2018.1134


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